منابع مشابه
Sulfhydryl Groups in Proteins
Egg albumin in the native, unaltered state, does not give tests characteristic of sulfhydryl groups. When, however, this protein is treated in any one of several ways, such as by heat (8, 9, 17), ultraviolet irradiation (lo), shaking (lo), or by solution in urea or other amides (7), free sulfhydryl groups make their appearance. The amount of free -SH groups appearing in egg albumin through the ...
متن کاملThe Reactions of the Sulfhydryl Groups of Human Hemoglobin &*
The rates at which the sulfhydryl groups on each chain of hemoglobin fi4 react with N-ethylmaleimide and iodoacetate have been measured. The sulfhydryl group at position 93 reacts with both alkylating agents at approximately the same rate as normal liganded hemoglobin. The sulfhydryl group at position 112, which is not available in normal hemoglobin, reacts in hemoglobin f14 and does so much mo...
متن کاملSulfhydryl Groups in Films of Egg Albumin
1. The same number of SH groups reduces ferricyanide in surface films of egg albumin as in albumin denatured by urea, guanidine hydrochloride, Duponol, or heat, provided the ferricyanide reacts with films while they still are at the surface and with the denatured proteins while the denaturing agent (urea, heat, etc.) is present. 2. The SH groups of a suspension of egg albumin made by clumping t...
متن کاملSulfhydryl Groups and the Structure of Hemoglobin
1. Addition of 2 moles of mersalyl, mercuric chloride, p-chloromercuribenzoate (PCMB), or methyl mercury hydroxide per mole of hemoglobin greatly reduces heme-heme interactions (n), yet these substances have quite different effects on the oxygen affinity (-log p(50)). Mersalyl and mercuric chloride at this concentration each increase the oxygen affinity, while PCMB and methyl mercury have littl...
متن کاملSulfhydryl and Disulfide Groups of Proteins
1. Methods have been described for reducing protein S-S groups, for oxidizing protein SH groups, and for estimating protein S-S and SH groups. 2. It has been found necessary in estimating the cystine content of proteins by the Folin-Marenzi method to take into account any cysteine that may be present. 3. A method for estimating the cysteine content of proteins has been described. 4. With these ...
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ژورنال
عنوان ژورنال: European Journal of Biochemistry
سال: 1969
ISSN: 0014-2956,1432-1033
DOI: 10.1111/j.1432-1033.1969.tb00687.x